Journal: Scientific reports
Article Title: Comparative in vitro immunoreactivity and protein analysis of Trimeresurus albolabris and Tropidolaemus wagleri venoms.
doi: 10.1038/s41598-025-97032-0
Figure Lengend Snippet: Fig. 2. Two-dimensional (2DE) separations and immunoblot analysis of Trimeresurus albolabris venom. 2DE gels stained with silver stain, spots were numbered, grouped and alphabetically listed from A–P (A). 2DE immunoblot of Trimeresurus albolabris proteins probed with hemato polyvalent antivenom (B). Identification and relative classification of immunoreactive proteins (C) and non-immunoreactive proteins (D) in Trimeresurus albolabris venom .
Article Snippet: The separated polypeptide spots from 2DE gels were transferred to nitrocellulose membrane for 90 min at 18 V on a Trans-blot semi-dry Transfer CellTM (Biorad) in semi-dry transfer buffer (48 mM Tris and 2.93 g glycine) pH 9.2 containing 20% methanol.
Techniques: Western Blot, Staining, Silver Staining